PRPF31

Clone Type

Polyclonal

Host

Goat

Source

Human

Isotype

IgG

Grade

Affinity Purified

Applications

E WB

Crossreactivity

Ca Hu

Accession #

NP_056444.3

Gene ID

26121

Shipping Temp

Blue Ice

Storage Temp

-20°C

Involved in pre-mRNA splicing. Required for U4/U6.U5 tri-snRNP formation.

Applications

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution

ELISA: 1:16,000 Western Blot: 0.3-1ug/ml, lysates of cell line A431 observed on ~60kD bands Optimal dilutions to be determined by the researcher.

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunogen

Synthetic peptide corresponding to C-LKVKGEKSGLMST, from human RP11, at C-terminal (NP_056444.3).

Form

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

Purity

Purified by immunoaffinity chromatography.

Specificity

Recognizes human RP11. Species sequence homology: Canine.

References

1. Makarova OV, Makarov EM, Liu S, Vornlocher HP, Luhrmann R. Protein 61K, encoded by a gene (PRPF31) linked to autosomal dominant retinitis pigmentosa, is required for U4/U6*U5 tri-snRNP formation and pre-mRNA splicing. EMBO J. 2002 Mar 1;21(5):1148-57.

USBio References

No references available

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P9121-40B Goat Anti-PRPF31 (U4/U6 Small Nuclear Ribonucleoprotein Prp31, Pre-mRNA-Processing Factor 31, Serologically Defined Breast Cancer Antigen NY-BR-99, U4/U6 snRNP 61kD Protein, Protein 61K, hPrp31, PRP31)

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