Transcriptional activator. Required for exocrine pancreatic development.
Applications
Suitable for use in ELISA. Other applications not tested.
Recommended Dilution
ELISA: 1:16,000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-EDPRKLHLKSSINN, from Ptf1a, at the internal region of the protein (NP_997524.1).
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes Ptf1a. Species sequence homology: Zebrafish.
References
1. Zecchin E, Mavropoulos A, Devos N, Filippi A, Tiso N, Meyer D, Peers B, Bortolussi M, Argenton F. Evolutionary conserved role of ptf1a in the specification of exocrine pancreatic fates. Dev Biol. 2004 Apr 1;268(1):174-84. Erratum in: Dev Biol. 2004 Jun 1;270(1):274.