2,3,6-Trimethyl-5-[(2-pyridinyldithio)methyl]-1H,7H-pyrazolo[1,2-a]pyrazole-1,7-dione;|PDT-Bimane
A fluorescent sulfhydryl active reagent.
Synonyms
2,3,6-Trimethyl-5-[(2-pyridinyldithio)methyl]-1H,7H-pyrazolo[1,2-a]pyrazole-1,7-dione; PDT-Bimane
Molecular Formula
C15H15N3O2S2
Appearance
Pale yellow solid
Method for Determining Identity
Proton NMR Spectroscopic and Mass Spectrometric Analysis
TLC Conditions
SiO2: Dichloromethane: Methanol; 9:1; Visualized with UV and AMCS, Rf = 0.5
Solubility
Chloroform (Slightly), DMSO (Slightly), Methanol (Slightly, Heated)
Storage and Stability
Store at 4°C. Light Sensitive. Amber vial. For maximum recovery of product, centrifuge the original vial prior to removing the cap.