ATP:Pyruvate 2-0-phosphotransferase; PK; PKM2, Pyruvate Kinase Isozymes M1/M2, Pyruvate Kinase Muscle Isozyme

9001-59-6

237kD (as a tetramer of four equal subunits 57kD)

≥200U/mg

As Reported

One unit will catalyze the conversion of 1.0 micromole of phosphoenolpyruvate to pyruvate per minute at pH 7.6 at 37°C

≥0.4mg protein/mg solid (A280nm, E0.1%=0.54)

CPK: ≤1% LDH: ≤0.01%

Enzymatic determination of ADP, ATP, pyruvate or phsphoenolpyruvate (PEP) when coupled with the related enzymes.

Optimal dilutions to be determined by the researcher.

Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with 0.9% saline. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Rabbit muscle

Not Determined

Supplied as lyophilized powder from Tris chloride, pH 7.7. Reconstitute with 0.9% saline

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.