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R1125-37 RAPD Kit (Random Amplified Polymorphic DNA), BioGenomics™, Kit-37

Specifications
References
Brand
BioGenomics™
Purity
Salt-free
Kit Type
PCR
Tests
2050
Detection Method
PCR
EU Commodity Code
38220000
Shipping Temp
Blue Ice
Storage Temp
-20°C

Each kit contains 20 individual 10-mer primers (supplied at a minimum amount of 50 nmole per primer). These primers are suitable for use in genetic mapping and DNA fingerprinting. In the RAPD technique, a single 10mer of arbitrary sequence is used as a primer in PCR to amplify genomic DNA where the sequence of the DNA is completely unknown. Genomic DNA from different individuals gives different PCR products, allowing the identification of DNA polymorphisms that can be used to identify different individuals or as genetic markers. The number of PCR products generated from each genomic DNA sample depends on the primer sequence, the genomic DNA sequence, and the genome size. Assuming that the priming sites of a 10mer RAPD primer are randomly distributed throughout a genome, the theoretical number of PCR products is approximately 2.5x10e9 xG, where G is the size of the haploid genome in base pairs. Using this calculation, a haploid genome of 2x10e9bp, for example, is expected to give about 5 PCR products. This prediction is in close agreement with experimental results.

Storage and Stability
Store at -20°C. Lyophilized powder is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Quality Control
Purity is verified by analytical PAGE.
GC Content
60-70% without self complementary ends.
Molecular Weight
As reported for each primer
Tm
As reported per primer
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. Grundmann, Towner, Dijkshoorn, Gerner-Smidt, Maher, Seifert, Vaneechoutte. Multicenter study using standardized protocols and reagents for evaluation of reproducibility af PCR-based fingerprinting of Acinetobacter spp. J. Clin. Microbiology 35(12): 3071-3077 (1997). 2. Berg, D., et al., Methods in Molecular and Cellular Biology 5: 13-24 (1994). 3. Welsh, J., McClelland, M., Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res. 18(24): 7213-7218 (1990). 4. Williams, J.G., Kubelik, A.R., Livak, K.J., Rafalski, J.A., Tingey, S.V., DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research 18: 6531-6535 (1990). 5. Micheli, M., et al. (1997). In Fingerprinting Methods Based on Arbitrarily Primed PCR, pp. 55-63. Springer-Verlag Press, NY. 6. Graves, L., Swaminathan, B. (1993). In Diagnostic Molecular Microbiology Principles and Applications, pp. 617-621. ASM Press, Washington, DC.
USBio References
No references available
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