Probable inhibitory regulator of the Ras-cyclic AMP pathway.
Applications
Suitable for use in Peptide ELISA and Western Blot. Other applications not tested.
Recommended Dilutions
Peptide ELISA: 1:32,000 Western Blot: 0.3-1ug/ml, observed in human thyroid gland lysates on ~90kD bands Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-QERGKAALGPLGP from human RASAL1, at C-terminal.
Form
Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human RASAL1.
References
1. Allen M, Chu S, Brill S, Stotler C, Buckler A. Restricted tissue expression pattern of a novel human rasGAP-related gene and its murine ortholog. Gene. 1998 Sep 18;218(1-2):17-25.