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R1293-03 Mouse Anti-Red Blood Cells (RBC)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Mouse
Isotype
IgG2a
Clone Number
13A66
Grade
Affinity Purified
Applications
FC
Crossreactivity
Mo
Shipping Temp
Blue Ice
Storage Temp
-20°C

The high-affinity anti-RBC monoclonal antibody efficiently bind to Fc receptors on macrophages inducing anemia in vivo due to a rapid Fc receptor (FcyR)-mediated erythrophagocytosis of opsonised RBC in spleen and livers. The capacity of the antibody to interact with FcyR is responsible for its haemolytic activity.

Applications
Suitable for use in Flow Cytometry and Functional Studies. Other applications not tested.
Recommended Dilution
Flow Cytometry: 1:50 Optimal dilutions to be determined by the researcher.
Positive Control: Mouse erythrocytes
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Mouse red blood cells
Form
Supplied as a liquid in PBS, 0.1% BSA.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes mouse RBC. Does not react with rat, sheep, rabit, chicken and human.
References
1. Shibata, T et al; Monoclonal anti-erythrocyte autoantibodies derived from NZB mice cause autoimmune haemolytic anemia by two distinct pathogenic mechanisms. Int. Immun. 1990, 2:1133. 2. Fossati-Jimack, L et al; High pathogenic potential of low-affinity autoantibodies in experimental autoimmune haemolytic anemia. J. Exp. Med. 1990, 190:1689. 3. Fossati-Jimack, L et al; Selective increase of autoimmune epitope expression on aged erythrocytes in mice: Implications in anti-erythrocyte autoimmune responses. J. Autoimmun. 2002, 18:17. 4. Azeredo da Silveira, S et al; Complement activation selectively potentiates the pathogenicity of the IgG2b and IgG3 isotypes of a high-affinity anti-erythrocyte autoantibody. J. Exp. Med. 2002, 195:665. 5. Baudino, L et al; Differential contribution of three different activating IgG Fc receptors (FcyRI, FcyRIII and FcyRIV) to IgG2a- and IgG2b-induced autoimmune haemolytic anemia in mice. J. Immunol. 2008, 180:1948.
USBio References
No references available
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