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R1998-12 Rhodamine, Dihydro 123, Dihydrochloride Salt (Dihydrorhodamine) CAS: 109244-58-8

Specifications
References
CAS Number
109244-58-8
Grade
Highly Purified
Molecular Formula
C₂₁H₁₈N₂O₃•2HCl
Molecular Weight
419
EU Commodity Code
38220090
Shipping Temp
RT
Storage Temp
-20°C
D 23806; D 632; DHR

Dihydrorhodamine 123 dihydrochloride is the HCl salt form of dihydrorhodamine 123 and is functionally equivalent to the latter. However, dihydrorhodamine 123 dihydrochloride is more water soluble and more stable to air oxidation than dihydrorhodamine 123. Thus, dihydrorhodamine 123 dihydrochloride is easier to use and can be stored longer than dihydrorhodamine 123.

Dihydrorhodamine 123 (DHR 123) is a cell-permeable fluorogenic probe that is useful for the detection of reactive oxygen species (ROS) such as peroxide and peroxynitrite. It is not fluorescent until oxidized by ROS to the highly fluorescent product Rhodamine 123. Neither NO, superoxide, nor hydrogen peroxide alone appear to oxidize DHR, rather, these ROS are thought to combine with other cellular components such as cytochrome c oxidase or Fe2+ in order to oxidize DHR 123 to its fluorescent derivative Rhodamine 123. Formation of Rhodamine 123 can be monitored by fluorescence spectroscopy using excitation and emission wavelengths of 500 and 536nm, respectively, or by absorbance spectroscopy at 500 nm (ε = 78,800 M-1cm-1).
Synonyms
D 23806; D 632; DHR
CAS No
109244-58-8
Molecular Formula
C₂₁H₁₈N₂O₃•2HCl
Molecular Weight
419
Purity
≥95% (HPLC and TLC)
Appearance
Off-white to pinkish solid
Absorption/Emission
505/534 nm (end product)
Solubility
Soluble in DMF or Water at ≥10mg/ml. Do not use DMSO
Storage and Stability
Powder is stable for 6 months after receipt at -20°C. Light sensitive.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Toxicity and Hazards
All products should be handled by qualified personnel only, trained in laboratory procedures.
References
1. J. Immunol. Meth. 178, 89(1995); 2. Eur. J. Biochem. 217, 973(1993); 3. Arc. Biochem. Biophys. 302, 348(1993); 4. Biochemistry 34, 3544(1995); 5. Free Rad. Biol Med. 16, 149(1994).
USBio References
No references available
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