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R8998-02A RPMI 1640 Medium w/L-Glutamine, HEPES, w/o Lysine

Specifications
References
Grade
Cell Culture Grade
EU Commodity Code
38210000
Shipping Temp
RT
Storage Temp
RT

RPMI-1640 was developed by Moore et. al. at Roswell Park Memorial Institute, hence the acronym RPMI. The formulation is based on the RPMI-1630 series of media utilizing a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. RPMI-1640 medium has been used for the culture of human normal and neoplastic leukocytes. RPMI-1640, when properly supplemented, has demonstrated wide applicability for supporting growth of many types of cultured cells, including fresh human lymphocytes in the 72 hour phytohemaglutinin (PHA) stimulation assay.

Appearance: Pale yellow to orange, homogeneous, free flowing powder
Solubility (14.9g/L): Orange, clear, complete
pH: As reported
Endotoxin: ≤10EU/mg
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Directions per Liter
Dissolve 14.95g in 800-900ml of ddH2O stirring gently until completely solubilized. Do NOT heat. If required, add sodium bicarbonate. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.
NOTE: It may be necessary to lower the pH to 4.0 with 1N HCl to completely dissolve this product. After it has dissolved completely, the pH can be raised to 7.2 with 1N NaOH prior to the addition of sodium bicarbonate.
Storage and Stability
Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Media Formulation
Components shown as mg/liter
Inorganic Salts
CalciumNitrate•4H2O100
MagnesiumSulfate48.84
PotassiumChloride400
SodiumChloride5300
SodiumPhosphatedibasic142
Amino Acids
L-Arginine200
L-Asparagine50
L-AsparticAcid20
L-Cystine•2HCl65
L-GlutamicAcid20
L-Glutamine300
Glycine10
L-Histidine15
Hydroxy-L-proline20
L-Isoleucine50
L-Leucine50
L-Methionine15
L-Phenylalanine15
L-Proline20
L-Serine30
L-Threonine20
L-Tryptophan5
L-Tyrosine•2Na•2H2O29
L-Valine20
Vitamins
p-AminobenzoicAcid1
D-Biotin0.2
CholineChloride3
VitaminB-120.005
FolicAcid1
myo-Inositol35
Niacinamide1
D-PantothenicAcid•Ca0.25
Pyridoxine•HCl1
Riboflavin0.2
Thiamine•HCl1
Other
D-Glucose2000
Glutathione(reduced)1
HEPES5958
PhenolRed,Sodium5
Total:14.952g/liter
References
1. Moore, G.E., Gerner, R.E., Franklin, H.A., JAMA 199: 519-524 (1967). 2. Moore, G.E., Woods L.K., Tissue Culture Association Manual 3: 503-508 (1976). 3. Moore, G.E., Gerner, R.E., Minowada, J., Twenty-First Annual Symposium on Fundamental Cancer Research. February: 41-63 (1967). 4. Moore, G.E., Gerner, R.E., Kitamura,H., NY State Journal of Medicine 68: 2054-2060 (1968).
USBio References
No references available
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