RPMI-1640 was developed by Moore et. al. at Roswell Park Memorial Institute, hence the acronym RPMI. The formulation is based on the RPMI-1630 series of media utilizing a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. RPMI-1640 medium has been used for the culture of human normal and neoplastic leukocytes. RPMI-1640, when properly supplemented, has demonstrated wide applicability for supporting growth of many types of cultured cells, including fresh human lymphocytes in the 72 hour phytohemaglutinin (PHA) stimulation assay.
Components shown as | mg/liter |
CalciumNitrate•4H2O | 100 |
MagnesiumSulfate | 48.84 |
PotassiumChloride | 400 |
SodiumChloride | 5300 |
SodiumPhosphatedibasic | 142 |
L-Arginine | 200 |
L-Asparagine | 50 |
L-AsparticAcid | 20 |
L-Cystine•2HCl | 65 |
L-GlutamicAcid | 20 |
L-Glutamine | 300 |
Glycine | 10 |
L-Histidine | 15 |
Hydroxy-L-proline | 20 |
L-Isoleucine | 50 |
L-Leucine | 50 |
L-Methionine | 15 |
L-Phenylalanine | 15 |
L-Proline | 20 |
L-Serine | 30 |
L-Threonine | 20 |
L-Tryptophan | 5 |
L-Tyrosine•2Na•2H2O | 29 |
L-Valine | 20 |
p-AminobenzoicAcid | 1 |
D-Biotin | 0.2 |
CholineChloride | 3 |
VitaminB-12 | 0.005 |
FolicAcid | 1 |
myo-Inositol | 35 |
Niacinamide | 1 |
D-PantothenicAcid•Ca | 0.25 |
Pyridoxine•HCl | 1 |
Riboflavin | 0.2 |
Thiamine•HCl | 1 |
D-Glucose | 2000 |
Glutathione(reduced) | 1 |
HEPES | 5958 |
PhenolRed,Sodium | 5 |
Total: | 14.952g/liter |
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