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R8999-04A RPMI 1640 Medium w/o Amino Acids, Sodium Phosphate (Powder)

Specifications
References
Grade
Cell Culture Grade
EU Commodity Code
38210000
Shipping Temp
RT
Storage Temp
RT/4°C

RPMI-1640 was developed by Moore, et al. at Roswell Park Memorial Institute, hence the acronym RPMI. The formulation is based on the RPMI-1630 series of media utilizing a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. RPMI-1640 medium has been used for the culture of human normal and neoplastic leukocytes. RPMI-1640, when properly supplemented, has demonstrated wide applicability for supporting growth of many types of cultured cells, including fresh human lymphocytes in the 72 hour phytohemaglutinin (PHA) stimulation assay.

Appearance
Yellow to orange, homogenous, free flowing powder
Solubility
Yellow, clear, complete
pH
As Reported
Endotoxin
≤1EU/ml
Directions per Liter
Dissolve 8.59g in 800-900ml of ddH2O stirring gently until completely solubilized. Add 2g/L sodium bicarbonate. Adjust pH of the medium to the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.
Storage and Stability
Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Media Formulation
Components shown as mg/liter
Inorganic Salts
Calcium Nitrate•4H2O100
Magnesium Sulfate48.84
Potassium Chloride400
Sodium Chloride6000
Vitamins
P-Aminobenzoic Acid1
D-Biotin0.2
Choline Chloride3
Cyanocobalamin0.005
Folic Acid1
myo-Inositol35
Niacinamide1
D-PantothenicAcid•Ca0.25
Pyridoxine•HCl1
Riboflavin0.2
Thiamine•HCl1
Other
D-Glucose2000
Glutathione1
Phenol Red, Sodium5.3
Total:8.59g/L
References
1. Flinn, R.J., et al., Mol. Biol. Cell. 21: 833-841 (2010). General Reference: 1. Moore, G.E., Gerner, R.E., Franklin, H.A., JAMA 199: 519-524 (1967). 2. Moore, G.E., Woods L.K., Tissue Culture Association Manual 3: 503-508. (1976). 3. Moore, G.E. Gerner, R.E., Minowada, J., Twenty-First Annual Symposium on Fundamental Cancer Research 41-63. (1967, February). 4. Moore, G.E. Gerner, R.E., Kitamura,H., NY State Journal of Medicine 68: 2054-2060 (1968).
USBio References
1. Chen X., et al. 2018. Whi2 is a conserved negative regulator of TORC1 in response to low amino acids. PLoS Genet. 14(8): e1007592.|2. Flinn, R.J., et al., 2010. Mol. Biol. Cell. 21: 833-841.
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