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R8999-14 RPMI 1640 Medium w/o L-Alanine, L-Glutamine, Folic acid, Riboflavin (Powder)

Specifications
References
Grade
Cell Culture Grade
EU Commodity Code
38210000
Shipping Temp
RT
Storage Temp
RT/4°C

RPMI-1640 was developed by Moore, et. al. at Roswell Park Memorial Institute, hence the acronym RPMI. The formulation is based on the RPMI-1630 series of media utilizing a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. RPMI-1640 medium has been used for the culture of human normal and neoplastic leukocytes. RPMI-1640, when properly supplemented, has demonstrated wide applicability for supporting growth of many types of cultured cells, including fresh human lymphocytes in the 72 hour phytohemaglutinin (PHA) stimulation assay.

Appearance
Light pink, homogenous, free flowing powder
Solubility
Pink. clear, complete
pH
As Reported
Endotoxin
≤1EU/ml
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Directions per Liter
Dissolve 10.092g in 900ml of ddH2O, stirring gently until completely solubilized. Do NOT heat. If required, add 2.0g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH units below the desired pH. Add additional water to bring the solution to final volume. Filter-sterilize using a 0.22 micron filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.
Note: It may be necessary to lower the pH to 4.0 with 1N HCl to completely dissolve this product. After it has dissolved completely, the pH can be raised to 7.2 with 1N NaOH prior to the addition of sodium bicarbonate..
Storage and Stability
Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Media Formulation
Components shown as g/L
Inorganic Salts
Calcium Nitrate•4H2O0.100
Magnesium Sulfate0.04884
Potassium Chloride0.400
Sodium Chloride6.000
Sodium Phosphate Dibasic0.800
Amino Acids
L-AlanineAbsent
L-Arginine0.200
L-Asparagine0.050
L-Aspartic Acid0.020
L-Cystine•2HCl0.065
L-Glutamic Acid0.020
L-GlutamineAbsent
Glycine0.010
L-Histidine0.015
Hydroxy-L-proline0.020
L-Isoleucine0.050
L-Leucine0.050
L-Lysine•HCl0.040
L-Methionine0.015
L-Phenylalanine0.015
L-Proline0.020
L-Serine0.030
L-Threonine0.020
L-Tryptophan0.005
L-Tyrosine•HCl0.028
L-Valine0.020
Vitamins
p-Aminobenzoic Acid0.001
D-Biotin0.0002
Choline Chloride0.003
Cyanocobalamin (Vit.B12)0.000005
Folic AcidAbsent
myo-Inositol0.035
Niacinamide0.001
D-Pantothenic Acid, Ca0.00025
Pyridoxal•HCl0.001
RiboflavinAbsent
Thiamine•HCl0.001
Other
D-Glucose2.000
Glutathione, reduced0.001
Phenol Red, Sodium0.0053
References
1. Moore, G.E., Gerner, R.E., Franklin, H.A., JAMA 199: 519-524 (1967). 2. Moore, G.E., Woods L.K., Tissue Culture Association Manual 3: 503-508. (1976). 3. Moore, G.E. Gerner, R.E., Minowada, J., Twenty-First Annual Symposium on Fundamental Cancer Research 41-63. (1967, February). 4. Moore, G.E. Gerner, R.E., Kitamura,H., NY State Journal of Medicine 68: 2054-2060 (1968).
USBio References
No references available
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