RPMI 1640 Medium w/o L-Alanine, L-Glutamine, Folic acid, Riboflavin

Grade

Cell Culture Grade

EU Commodity Code

38210000

Shipping Temp

Storage Temp

RT/4°C

RPMI-1640 was developed by Moore, et. al. at Roswell Park Memorial Institute, hence the acronym RPMI. The formulation is based on the RPMI-1630 series of media utilizing a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. RPMI-1640 medium has been used for the culture of human normal and neoplastic leukocytes. RPMI-1640, when properly supplemented, has demonstrated wide applicability for supporting growth of many types of cultured cells, including fresh human lymphocytes in the 72 hour phytohemaglutinin (PHA) stimulation assay.

Appearance

Light pink, homogenous, free flowing powder

Solubility

Pink. clear, complete

As Reported

Endotoxin

≤1EU/ml

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Directions per Liter

Dissolve 10.092g in 900ml of ddH2O, stirring gently until completely solubilized. Do NOT heat. If required, add 2.0g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH units below the desired pH. Add additional water to bring the solution to final volume. Filter-sterilize using a 0.22 micron filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Note: It may be necessary to lower the pH to 4.0 with 1N HCl to completely dissolve this product. After it has dissolved completely, the pH can be raised to 7.2 with 1N NaOH prior to the addition of sodium bicarbonate..

Storage and Stability

Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Media Formulation

Components shown as g/L

Inorganic Salts

Calcium Nitrate•4H2O	0.100
Magnesium Sulfate	0.04884
Potassium Chloride	0.400
Sodium Chloride	6.000
Sodium Phosphate Dibasic	0.800

Amino Acids

L-Alanine	Absent
L-Arginine	0.200
L-Asparagine	0.050
L-Aspartic Acid	0.020
L-Cystine•2HCl	0.065
L-Glutamic Acid	0.020
L-Glutamine	Absent
Glycine	0.010
L-Histidine	0.015
Hydroxy-L-proline	0.020
L-Isoleucine	0.050
L-Leucine	0.050
L-Lysine•HCl	0.040
L-Methionine	0.015
L-Phenylalanine	0.015
L-Proline	0.020
L-Serine	0.030
L-Threonine	0.020
L-Tryptophan	0.005
L-Tyrosine•HCl	0.028
L-Valine	0.020

Vitamins

p-Aminobenzoic Acid	0.001
D-Biotin	0.0002
Choline Chloride	0.003
Cyanocobalamin (Vit.B12)	0.000005
Folic Acid	Absent
myo-Inositol	0.035
Niacinamide	0.001
D-Pantothenic Acid, Ca	0.00025
Pyridoxal•HCl	0.001
Riboflavin	Absent
Thiamine•HCl	0.001

Other

D-Glucose	2.000
Glutathione, reduced	0.001
Phenol Red, Sodium	0.0053

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R8999-14 RPMI 1640 Medium w/o L-Alanine, L-Glutamine, Folic acid, Riboflavin (Powder)

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