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R9002-01 RPMI 1640 Medium Modified w/L-Glutamine w/o Phenol Red, Biotin (Powder)

Specifications
References
Grade
Cell Culture Grade
EU Commodity Code
38210000
Shipping Temp
RT
Storage Temp
RT/4°C

Appearance
White to yellow, homogenous free flowing powder
Solubility
Colorless to light yellow, clear, complete
pH
As Reported
Endotoxin
≤1EU/ml
Directions per Liter
Dissolve 10..4g in 900ml of ddH2O, stirring until completely solubilized. Do NOT heat. Add 2g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH units below the desired pH. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22um membrane. Aliquot into sterile containers.
Note
It may be necessary to lower the pH to 4.0 with 1N HCl to completely dissolve this product. After it has dissolved completely, the pH can be raised to 7.2 with 1N NaOH prior to the addition of sodium bicarbonate.
Storage and Stability
Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Media Formulation
Components shown as g/liter
Inorganic Salts
Calcium Nitrate•4H2O0.1
Magnesium Sulfate0.04884
Potassium Chloride0.4
Sodium Chloride6
Sodium Phosphate Dibasic0.8
Amino Acids
L-Arginine0.2
L-Asparagine (anhydrous)0.05
L-Aspartic Acid0.02
L-Cystine•2HCl0.0652
L-Glutamic Acid0.02
L-Glutamine0.3
Glycine0.01
L-Histidine0.015
Hydroxy-L-proline0.02
L-Isoleucine0.05
L-Leucine0.05
L-Lysine•HCl0.04
L-Methionine0.015
L-Phenylalanine0.015
L-Proline0.02
L-Serine0.03
L-Threonine0.02
L-Tryptophan0.005
L-Tyrosine•2Na•2H2O0.02883
L-Valine0.02
Vitamins
P-Amino Benzoic Acid0.001
D-BiotinAbsent
Choline Chloride0.003
Vitamin B-120.000005
Folic Acid0.001
myo-Inositol0.035
Niacinamide0.001
D-Pantothenic Acid•Ca0.00025
Pyridoxine•HCl0.001
Riboflavin0.0002
Thiamine•HCl0.001
Other
D-Glucose2
Glutathione0.001
Phenol RedAbsent
Total:10.4g/Liter
References
1. Moore, G.E., Gerner, R.E. and Franklin, H.A., JAMA. 199, 519-524. (1967). 2. Moore, G.E. and Woods L.K., Tissue Culture Association Manual, 3, 503-508. (1976). 3. Moore, G.E. Gerner, R.E. and Minowada, J., Twenty-First Annual Symposium on Fundamental Cancer Research. February, 41-63. (1967). 4. Moore, G.E. Gerner, R.E. and Kitamura,H.,. NY State Journal of Medicine. 68, 2054-2060. (1968)
USBio References
No references available
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