RPMI 1640 Medium Modified w/L-Glutamine w/o Phenol Red, Biotin

Grade

Cell Culture Grade

EU Commodity Code

38210000

Shipping Temp

Storage Temp

RT/4°C

Appearance

White to yellow, homogenous free flowing powder

Solubility

Colorless to light yellow, clear, complete

As Reported

Endotoxin

≤1EU/ml

Directions per Liter

Dissolve 10..4g in 900ml of ddH2O, stirring until completely solubilized. Do NOT heat. Add 2g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH units below the desired pH. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22um membrane. Aliquot into sterile containers.

Note

It may be necessary to lower the pH to 4.0 with 1N HCl to completely dissolve this product. After it has dissolved completely, the pH can be raised to 7.2 with 1N NaOH prior to the addition of sodium bicarbonate.

Storage and Stability

Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Media Formulation

Components shown as g/liter

Inorganic Salts

Calcium Nitrate•4H2O	0.1
Magnesium Sulfate	0.04884
Potassium Chloride	0.4
Sodium Chloride	6
Sodium Phosphate Dibasic	0.8

Amino Acids

L-Arginine	0.2
L-Asparagine (anhydrous)	0.05
L-Aspartic Acid	0.02
L-Cystine•2HCl	0.0652
L-Glutamic Acid	0.02
L-Glutamine	0.3
Glycine	0.01
L-Histidine	0.015
Hydroxy-L-proline	0.02
L-Isoleucine	0.05
L-Leucine	0.05
L-Lysine•HCl	0.04
L-Methionine	0.015
L-Phenylalanine	0.015
L-Proline	0.02
L-Serine	0.03
L-Threonine	0.02
L-Tryptophan	0.005
L-Tyrosine•2Na•2H2O	0.02883
L-Valine	0.02

Vitamins

P-Amino Benzoic Acid	0.001
D-Biotin	Absent
Choline Chloride	0.003
Vitamin B-12	0.000005
Folic Acid	0.001
myo-Inositol	0.035
Niacinamide	0.001
D-Pantothenic Acid•Ca	0.00025
Pyridoxine•HCl	0.001
Riboflavin	0.0002
Thiamine•HCl	0.001

Other

D-Glucose	2
Glutathione	0.001
Phenol Red	Absent

Total:

10.4g/Liter

References

1. Moore, G.E., Gerner, R.E. and Franklin, H.A., JAMA. 199, 519-524. (1967). 2. Moore, G.E. and Woods L.K., Tissue Culture Association Manual, 3, 503-508. (1976). 3. Moore, G.E. Gerner, R.E. and Minowada, J., Twenty-First Annual Symposium on Fundamental Cancer Research. February, 41-63. (1967). 4. Moore, G.E. Gerner, R.E. and Kitamura,H.,. NY State Journal of Medicine. 68, 2054-2060. (1968)

USBio References

No references available

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R9002-01 RPMI 1640 Medium Modified w/L-Glutamine w/o Phenol Red, Biotin (Powder)

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