RPMI 1640 Medium Modified w/o L-Glutamine, w/o Amino acids, Glucose

Grade

Cell Culture Grade

EU Commodity Code

38210000

Shipping Temp

Storage Temp

RT/4°C

RPMI 1640 is used for the culture of human normal and neoplastic leukocytes. RPMI 1640 was developed by Moore et al. at Roswell Park Memorial Institute.

Appearance

Yellow to light orange, homogenous, free flowing powder

Solubility

Magenta, clear, complete

As Reported

Endotoxin

≤1EU/ml

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Directions per Liter

Dissolve 7.40g in 900ml of ddH2O, stirring until completely solubilized. Do NOT heat. Add 2g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH units below the desired pH. Add additional water to bring the solution to final volume. Filter-sterilize using a 0.22um membrane. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability

Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Media Formulation

Components shown as g/liter

Inorganic Salts

Calcium Nitrate•4H2O	0.1
Magnesium Sulfate	0.04884
Potassium Chloride	0.4
Sodium Chloride	6
Sodium Phosphate Dibasic	0.8

Amino Acids

L-Arginine	Absent
L-Aspartic Acid	Absent
L-Asparagine	Absent
L-Cystine•2HCl	Absent
L-Glutamic Acid	Absent
L-Glutamine	Absent
Glycine	Absent
L-Histidine	Absent
Hydroxy-L-proline	Absent
L-Isoleucine	Absent
L-Leucine	Absent
L-Lysine•HCl	Absent
L-Methionine	Absent
L-Phenylalanine	Absent
L-Proline	Absent
L-Serine	Absent
L-Threonine	Absent
L-Tryptophan	Absent
L-Tyrosine•2Na•2H2O	Absent
L-Valine	Absent

Vitamins

P-Amino Benzoic Acid	0.001
D-Biotin	0.0002
Choline Chloride	0.003
Vitamin B-12	0.000005
Folic Acid	0.001
myo-Inositol	0.035
Niacinamide	0.001
D-Pantothenic Acid•Ca	0.00025
Pyridoxine•HCl	0.001
Riboflavin	0.0002
Thiamine•HCl	0.001

Other

Glucose	Absent
Glutathione	0.001
Phenol Red, Sodium	0.0053

Total

7.40g/liter

References

1. Moore, G.E., Gerner, R.E. and Franklin, H.A., JAMA. 199, 519-524. (1967). 2. Moore, G.E. and Woods L.K., Tissue Culture Association Manual, 3, 503-508. (1976). 3. Moore, G.E. Gerner, R.E. and Minowada, J., Twenty-First Annual Symposium on Fundamental Cancer Research. February, 41-63. (1967). 4. Moore, G.E. Gerner, R.E. and Kitamura,H.,. NY State Journal of Medicine. 68, 2054-2060. (1968)

USBio References

1. Elghazi L., et al. 2017. Role of nutrients and mTOR signaling in the regulation of pancreatic progenitors development. Mol Metab. 6(6):560-573. doi: 10.1016/j.molmet.2017.03.010. |2. Medina AB, et al. 2017. ρ0 Cells Feature De-Ubiquitination of SLC Transporters and Increased Levels and Fluxes of Amino Acids. Int J Mol Sci. 18(4):879. doi:10.3390/ijms18040879

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R9010-01 RPMI 1640 Medium Modified w/o L-Glutamine, w/o Amino acids, Glucose (Powder)

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