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R9010-02 RPMI 1640 Medium Modified w/o L-Glutamine, w/o Amino acids, Glucose, Phenol Red (Powder)

Specifications
References
Grade
Cell Culture Grade
EU Commodity Code
38210000
Shipping Temp
RT
Storage Temp
RT/4°C

RPMI-1640 was developed by Moore et. al. at Roswell Park Memorial Institute, hence the acronym RPMI. 

Appearance
White to light yellow homogenous, free flowing powder
Solubility
Colorless, clear, complete
pH
As Reported
Endotoxin
≤1EU/ml
Directions per Liter
Dissolve 7.4 grams in 900ml of ddH2O stirring gently until completely solubilized. Do NOT heat. If required, add 2g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired pH. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane. Aliquot into sterile containers.
Storage and Stability
Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Media Formulation
Components shown as g/liter
Inorganic Salts
Calcium Nitrate•4H2O0.1
Magnesium Sulfate0.04884
Potassium Chloride0.4
Sodium Chloride6
Sodium Phosphate Dibasic0.8
Amino Acids
L-ArginineAbsent
L-Aspartic AcidAbsent
L-AsparagineAbsent
L-Cystine•2HClAbsent
L-Glutamic AcidAbsent
L-GlutamineAbsent
GlycineAbsent
L-HistidineAbsent
Hydroxy-L-prolineAbsent
L-IsoleucineAbsent
L-LeucineAbsent
L-Lysine•HClAbsent
L-MethionineAbsent
L-PhenylalanineAbsent
L-ProlineAbsent
L-SerineAbsent
L-ThreonineAbsent
L-TryptophanAbsent
L-Tyrosine•2Na•2H2OAbsent
L-ValineAbsent
Vitamins
P-Amino Benzoic Acid0.001
D-Biotin0.0002
Choline Chloride0.003
Vitamin B-120.000005
Folic Acid0.001
myo-Inositol0.035
Niacinamide0.001
D-Pantothenic Acid•Ca0.00025
Pyridoxine•HCl0.001
Riboflavin0.0002
Thiamine•HCl0.001
Other
GlucoseAbsent
Glutathione0.001
Phenol Red, SodiumAbsent
Total7.4g/liter
References
1. Moore, G.E., Gerner, R.E., Franklin, H.A., JAMA 199: 519-524 (1967). 2. Moore, G.E., Woods L.K., Tissue Culture Association Manual 3: 503-508 (1976). 3. Moore, G.E., Gerner, R.E., Minowada, J., Twenty-First Annual Symposium on Fundamental Cancer Research. February: 41-63 (1967). 4. Moore, G.E., Gerner, R.E., Kitamura,H., NY State Journal of Medicine 68: 2054-2060 (1968).
USBio References
No references available
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