RPMI 1640 Medium Modified w/o L-Glutamine, w/o Amino acids, Glucose, Phenol Red

Grade

Cell Culture Grade

EU Commodity Code

38210000

Shipping Temp

Storage Temp

RT/4°C

RPMI-1640 was developed by Moore et. al. at Roswell Park Memorial Institute, hence the acronym RPMI.

Appearance

White to light yellow homogenous, free flowing powder

Solubility

Colorless, clear, complete

As Reported

Endotoxin

≤1EU/ml

Directions per Liter

Dissolve 7.4 grams in 900ml of ddH2O stirring gently until completely solubilized. Do NOT heat. If required, add 2g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired pH. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane. Aliquot into sterile containers.

Storage and Stability

Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Media Formulation

Components shown as g/liter

Inorganic Salts

Calcium Nitrate•4H2O	0.1
Magnesium Sulfate	0.04884
Potassium Chloride	0.4
Sodium Chloride	6
Sodium Phosphate Dibasic	0.8

Amino Acids

L-Arginine	Absent
L-Aspartic Acid	Absent
L-Asparagine	Absent
L-Cystine•2HCl	Absent
L-Glutamic Acid	Absent
L-Glutamine	Absent
Glycine	Absent
L-Histidine	Absent
Hydroxy-L-proline	Absent
L-Isoleucine	Absent
L-Leucine	Absent
L-Lysine•HCl	Absent
L-Methionine	Absent
L-Phenylalanine	Absent
L-Proline	Absent
L-Serine	Absent
L-Threonine	Absent
L-Tryptophan	Absent
L-Tyrosine•2Na•2H2O	Absent
L-Valine	Absent

Vitamins

P-Amino Benzoic Acid	0.001
D-Biotin	0.0002
Choline Chloride	0.003
Vitamin B-12	0.000005
Folic Acid	0.001
myo-Inositol	0.035
Niacinamide	0.001
D-Pantothenic Acid•Ca	0.00025
Pyridoxine•HCl	0.001
Riboflavin	0.0002
Thiamine•HCl	0.001

Other

Glucose	Absent
Glutathione	0.001
Phenol Red, Sodium	Absent

Total

7.4g/liter

References

1. Moore, G.E., Gerner, R.E., Franklin, H.A., JAMA 199: 519-524 (1967). 2. Moore, G.E., Woods L.K., Tissue Culture Association Manual 3: 503-508 (1976). 3. Moore, G.E., Gerner, R.E., Minowada, J., Twenty-First Annual Symposium on Fundamental Cancer Research. February: 41-63 (1967). 4. Moore, G.E., Gerner, R.E., Kitamura,H., NY State Journal of Medicine 68: 2054-2060 (1968).

USBio References

No references available

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R9010-02 RPMI 1640 Medium Modified w/o L-Glutamine, w/o Amino acids, Glucose, Phenol Red (Powder)

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