Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Not suitable for use in Flow Cytometry. Other applications not tested.

Western Blot: Detects native SERPINB9 at 4~2kD as well as SERPINB9 complexed to granzyme B at ~67kD. As a positive control for SERPINB9 , YT-Indy cells (a natural killer leukemia cell line) can be used. In other cell lines a non-specific protein band of ~63kD can be detected. Immunohistochemistry: 1:100 Can be used on formaldehyde-fixed paraffin-embedded human tissues after pretreatment with 10mM citric acid pH 6.0 for 20 min. Immunocytochemistry: Fix cells for 10 min in 10% formalin and then treat them as paraffin sections. Optimal dilutions to be determined by the researcher.

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Full length recombinant SERPINB9 produced in E. coli.

Supplied as a liquid in 0.7% BSA, 0.09% sodium azide.

Supernatant

Recognizes human SERPINB9.