Suitable for use in Western Blot, Immunocytochemistry and Immunohistochemistry. Other applications not tested.

Western Blot: 0.1-0.2ug/ml; The detection limit for recombinant mouse SFRP3 is ~5ng/lane under non-reducing and reducing conditions. In this format, shows ~60% crossreactivity with recombinant human SFRP3. Immunohistochemistry: 2-5ug/ml; Detects SFRP3 in cells and tissues. Optimal dilutions to be determined by the researcher.

Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile TBS, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Recombinant protein corresponding to Ala33-Ser323 from mouse SFRP3, expressed in NS0 cells.

Supplied as a lyophilized powder from PBS, BSA. Labeled with Biotin. Reconstitute with 1ml sterile TBS, pH 7.3, 0.1% BSA.

Purified by immunoaffinity chromatography.

Recognizes mouse SFRP3.