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S1013-02 Shields and Sang M3 Insect Medium w/ L-Glutamine, w/o Glucose (Powder)

Specifications
References
Grade
Cell Culture Grade
EU Commodity Code
38210000
Shipping Temp
RT
Storage Temp
RT/4°C

Shields and Sang M3 Insect medium supports the rapid growth of both primary and established cultures of Drosophila cell lines.

Appearance
White to off white, homogeneous, free flowing powder
Solubility
Colorless to light yellow, clear, complete
pH
As Reported
Directions per Liter
Dissolve 29.86 grams in 900ml of ddH2O, stirring gently until completely solubilized. Adjust pH of the medium to 0.1-0.3 pH units below the desired pH. Add additional water to bring the solution to final volume of 1L. Filter-sterilize using a 0.22 micron membrane. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.
Storage and Stability
Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Media Formulation
Components shown as g/L
Inorganic Salts
Calcium Chloride 0.760
Magnesium Sulfate 2.149
Potassium Bicarbonate0.500
Sodium Phosphate Monobasic0.880
Amino Acids
ß-Alanine0.250
L-Alanine1.500
L-Arginine0.500
L-Asparagine 0.300
L-Aspartic Acid0.300
L-Cysteine•HCl0.200
L-Glutamic Acid•K7.880
L-Glutamic Acid•Na6.530
L-Glutamine0.600
Glycine0.500
L-Histidine0.550
L-Isoleucine0.250
L-Leucine0.400
L-Lysine•HCl0.850
L-Methionine0.250
L-Phenylalanine0.250
L-Proline0.400
L-Serine0.350
L-Threonine0.500
L-Tryptophan0.100
L-Tyrosine•2Na0.360
L-Valine0.400
Vitamins
Choline Chloride0.050
Oxalacetic Acid0.250
Other
D(+)-GlucoseAbsent
BIS-TRIS1.050
Yeast Extract1.000
Total: 29.86g/L
References
1. Shields, G., Sang, J.H.: Improved medium for culture of Drosophila embryonic cells. Drosophila Information Service 52: 161 (1977). 2. Shields, G., Dubendorfer, A., Sang, J.H.: Differentiation in vitro of larval cells from early embryonic cells of Drosophila melangaster. J. Embryol. Exp. Morphol. 33: 159-175 (1975).
USBio References
No references available
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