Shields and Sang M3 Insect Medium w/ L-Glutamine, w/o Glucose

Grade

Cell Culture Grade

EU Commodity Code

38210000

Shipping Temp

Storage Temp

RT/4°C

Shields and Sang M3 Insect medium supports the rapid growth of both primary and established cultures of Drosophila cell lines.

Appearance

White to off white, homogeneous, free flowing powder

Solubility

Colorless to light yellow, clear, complete

As Reported

Directions per Liter

Dissolve 29.86 grams in 900ml of ddH2O, stirring gently until completely solubilized. Adjust pH of the medium to 0.1-0.3 pH units below the desired pH. Add additional water to bring the solution to final volume of 1L. Filter-sterilize using a 0.22 micron membrane. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability

Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Media Formulation

Components shown as g/L

Inorganic Salts

Calcium Chloride	0.760
Magnesium Sulfate	2.149
Potassium Bicarbonate	0.500
Sodium Phosphate Monobasic	0.880

Amino Acids

ß-Alanine	0.250
L-Alanine	1.500
L-Arginine	0.500
L-Asparagine	0.300
L-Aspartic Acid	0.300
L-Cysteine•HCl	0.200
L-Glutamic Acid•K	7.880
L-Glutamic Acid•Na	6.530
L-Glutamine	0.600
Glycine	0.500
L-Histidine	0.550
L-Isoleucine	0.250
L-Leucine	0.400
L-Lysine•HCl	0.850
L-Methionine	0.250
L-Phenylalanine	0.250
L-Proline	0.400
L-Serine	0.350
L-Threonine	0.500
L-Tryptophan	0.100
L-Tyrosine•2Na	0.360
L-Valine	0.400

Vitamins

Choline Chloride	0.050
Oxalacetic Acid	0.250

Other

D(+)-Glucose	Absent
BIS-TRIS	1.050
Yeast Extract	1.000

Total:

29.86g/L

References

1. Shields, G., Sang, J.H.: Improved medium for culture of Drosophila embryonic cells. Drosophila Information Service 52: 161 (1977). 2. Shields, G., Dubendorfer, A., Sang, J.H.: Differentiation in vitro of larval cells from early embryonic cells of Drosophila melangaster. J. Embryol. Exp. Morphol. 33: 159-175 (1975).

USBio References

No references available

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S1013-02 Shields and Sang M3 Insect Medium w/ L-Glutamine, w/o Glucose (Powder)

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