Suitable for use in Immunoblot and Immunoprecipitation. Other applications not tested.

Immunoblot Analysis: 0.5-2ug/ml Detects SHIP in RIPA lysates from human TF-1 cells (a bone marrow erythroleukemia cell line). TF-1 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with S1013-20A (2ug/ml). Proteins were visualized using a goat anti-rabbit IgG labeled with HRP and a chemiluminescence detection system. Immunoprecipitation: 2ug Reported to immunoprecipitate SHIP from Ramos cells (human B cell line). Optimal dilution determined by the researcher.

May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Fusion protein corresponding to aa7-133 of murine SHIP SH2 domain.

Supplied as a liquid in 0.07M Tris, pH 7.4, 0.105M sodium chloride. No stabilizing proteins added. No preservative added. Also available with BSA & Azide. See S1013-20.

Purified by Protein A affinity chromatography.

Recognizes SHIP, 145kD and/or 135kD as well as C-terminal truncated forms at 125kD and 110kD in some cell types. Species Crossreactivity: human and mouse.