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S4283-03 Rabbit Anti-SPAK, CT (STE20/SPS1-related Proline-alanine-rich Protein Kinase, Ste-20-related Kinase, Serine/Threonine-protein Kinase 39, DCHT, STK39, SPAK)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Swiss Prot
Q9UEW8
Isotype
IgG
Grade
Purified
Applications
E IHC WB
Crossreactivity
Hu Mo
Accession #
NP_037365
Shipping Temp
Blue Ice
Storage Temp
-20°C

STK39 is a serine/threonine kinase that is thought to function in the cellular stress response pathway. The kinase is activated in response to hypotonic stress, leading to phosphorylation of several cation-chloride-coupled cotransporters. The catalytically active kinase specifically activates the p38 MAP kinase pathway, and its interaction with p38 decreases upon cellular stress, suggesting that this kinase may serve as an intermediate in the response to cellular stress.

Applications
Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.
Recommended Dilution
ELISA: 1:1,000 Western Blot: 1:100-1:500 Immunohistochemistry: 1:10-1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide selected from the C-terminal region of human STK39 (KLH).
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by ammonium sulfate precipitation
Specificity
Recognizes human STK39. Species Crossreactivity: mouse.
References
1. Dowd, B.F., et al., J. Biol. Chem. 278(30):27347-27353 (2003). 2. Johnston, A.M., et al., Oncogene 19(37):4290-4297 (2000).
USBio References
No references available
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