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S9090 SMWTY Marine Medum (ATCC 231)

Specifications
References
Grade
Microbiological Grade
EU Commodity Code
38210000
Shipping Temp
RT
Storage Temp
RT/4°C

Used for the cultivation and maintenance of a variety of heterotrophic marine bacterial species.

Part A
Appearance: White to light yellow, homogeneous, free flowing powder.
Solubility: Colorless to light yellow, clear, complete
pH: As Reported
Trace Elements (Part B)
Appearance: White, homogeneous, free flowing powder.
Solubility: Colorless, clear, complete
pH: As Reported
Directions per Liter
Dissolve 5.6 grams of Part A in 800-900ml of seawater stirring gently with heating until completely solubilized. Dissolve 7.9 grams of Part B in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Add additional water to bring Part B to 1L. Take 1ml of Part B Solution and add to Part A Solution. Add additional water to Part A Solution to bring to 1L. Adjust to a final pH of 7.0. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).
Storage and Stability
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.
Media Formulation
ATCC medium: 231 SWMTY - marine medium
Part A
Potassium Nitrate0.5g
Sodium glycerophosphate0.1g
Trace Elements (see below)1.0ml
Tris buffer1.0g
Tryptone2.0g
Yeast extract2.0g
Trace Elements (Part B)
Boric Acid (H3BO3)2.85g
Cobalt Chloride•6H2O40.4mg
Copper Chloride•2H2O26.9mg
Ferric Sulfate1.36g
Manganese Chloride•4H2O1.8g
Sodium Molybdate•2H2O25.2mg
Sodium tartrate1.77g
Seawater1.0L
Zinc Chloride20.8mg
References
Handbook of Microbiological Media By Ronald M. Atlas
USBio References
No references available
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