USBio Logo

S9100-01X Mouse Anti-SYK, phosphorylated (Y525/Y526) (Spleen Tyrosine Kinase, HGNC:11491, p72Syk, Tyrosine Protein Kinase SYK)

Specifications
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG2b
Clone Number
11D111
Grade
Affinity Purified
Applications
WB
Crossreactivity
Hu
Gene ID
6850
Shipping Temp
Blue Ice
Storage Temp
-20°C

Spleen tyrosine kinase (SYK; also tyrosine-protein kinase SYK) is a 70-80 kDa, ubiquitously expressed member of the protein kinase superfamily, the tyrosine protein kinase family, and the SYK/ZAP-70 subfamily of proteins. Human SYK is 635 amino acids (aa) in length and contains two SH2 domains (aa 14-106 and aa 168-259), and one protein kinase domain (aa 371-631). A splicing variant produces a second isoform that has a deletion of aa 371-631 found in the longer isoform. Human SYK shares 92% and 91% aa sequence identity with mouse and rat SYK, respectively. Functionally, SYK is a positive effector of B-cell antigen receptor-stimulated responses.

Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilutions
Western Blot: 0.1ug/ml detects human SYK phosphorylated at Y525/Y526 in Ramos human Burkitt’s lymphoma cell line treated with IgM and U937 human histiocytic lymphoma cell line treated with pervanadate. Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Phosphopeptide containing human SYK Y525/Y526 sites.
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Purity
Purified by Protein A affinity chromatography from hybridoma culture supernatant.
Specificity
Recognizes human SYK when phosphorylated at Y525/Y526.
Conjugates
Pricing
Order
Proceed to Checkout
Cart Summary
ProductSizeListYour PriceQtyExt Price
Subtotal:Subtotal:
Subtotal:Subtotal:
Total Coupon Savings:Total Coupon Savings:()
Your cart is currently empty.
- Coupon Code
Recently Viewed
Contact Us

Visit our technical library or contact our support staff to answer your questions.

Telephone:
1-800-520-3011

Library | Contact

Distributors

For customers outside of the United States, please use one of our many distributors.

View Distributors

Payment Methods

We accept the following payment methods as well as pay-by-invoice.

MasterCard Visa PayPal
© 2023-2024 United States Biological - All Rights Reserved