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S9270-01 Synthetic Amino Acid Medium, Bacteriological, SAAM-B w/o Phenol Red w/o Riboflavin (Powder)

Specifications
Grade
Molecular Biology Grade
EU Commodity Code
38210000
Shipping Temp
RT
Storage Temp
RT/4°C

Appearance
Off-white to pale yellow, homogeneous, free flowing powder.
Solubility
Colorless to pale yellow, slight precipitate
pH: As Reported
Directions per Liter
Dissolve 32g in 800-900ml of ddH2O stirring gently until completely solubilized. If required add sodium bicarbonate. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L Filter-sterilize using a 0.22um membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.
Storage and Stability
Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Media Formulation
Components shown as mg/liter
Inorganic Salts
Calcium Chloride•2H2O8.20
Ferric Chloride•6H2O2.69
Magnesium Chloride95.35
Manganese Sulfate•4H2O0.36
Potassium Phosphate Dibasic381.70
Zinc Sulfate•7H2O0.80
Amino Acids
L-Arginine HCl1270.00
L-Asparagine•H2O1136.00
L-Cysteine•HCl•H2O1450.00
L-Cystine221.70
L-Glutamine2520.00
Glycine500.00
L-Histidine•HCl•H2O419.00
L-Isoleucine520.00
L-Leucine520.00
L-Lysine•HCI724.65
L-Methionine150.00
L-Phenylalanine320.00
L-Proline1000.00
L-Threonine480.00
L-Tryptophan100.00
L-Tyrosine•Na2•2H2O519.00
L-Valine460.00
Vitamins
Biotin0.50
Choline Chloride25.00
Folic Acid5.00
i-Inositol25.00
Nicotinamide5.00
D-Pantothenic Acid•Ca25.00
Pyridoxal•HCI5.00
Riboflavinabsent
Thiamine•HCI5.00
Other
Adenine25.00
Ammonium Acetate500.00
Fumaric Acid1500.00
D-Glucose1000.00
MOPS•Na15030.00 (65mM)
Sodium Pyruvate1000.00
Uracil25.00
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