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T1033-02 Tau, Recombinant, Human (Microtubule-associated Protein Tau Isoform 3, Neurofibrillary Tangle Protein, Paired Helical Filament-tau, PHF-tau, MAPT, MAPTL, MTBT1, TAU) CAS:

Specifications
References
Grade
Affinity Purified
Accession Number
NP_058518
Molecular Weight
42.1
EU Commodity Code
30021019
Shipping Temp
Blue Ice
Storage Temp
-20°C

Tau, also known as microtubule-associated protein tau (MAPT), is a protein that stabilizes microtubules. It is abundant in neurons in the central nervous system and is less common elsewhere. When this protein is defective, and no longer stabilizes microtubules properly, it can result in dementias, such as Alzheimer's disease.

Source
Recombinant corresponding to aa1-383 of human Tau protein, fused to His-tag at N-terminus, expressed in E.coli.
AA Sequence
MGSSHHHHHH SSGLVPRGSH MAEPRQEFEV MEDHAGTYGL GDRKDQGGYT MHQDQEGDTD AGLKAEEAGI GDTPSLEDEA AGHVTQARMV SKSKDGTGSD DKKAKGADGK TKIATPRGAA PPGQKGQANA TRIPAKTPPA PKTPPSSGEP PKSGDRSGYS SPGSPGTPGS RSRTPSLPTP PTREPKKVAV VRTPPKSPSS AKSRLQTAPV PMPDLKNVKS KIGSTENLKH QPGGGKVQII NKKLDLSNVQ SKCGSKDNIK HVPGGGSVQI VYKPVDLSKV TSKCGSLGNI HHKPGGGQVE VKSEKLDFKD RVQSKIGSLD NITHVPGGGN KKIETHKLTF RENAKAKTDH GAEIVYKSPV VSGDTSPRHL SNVSSTGSID MVDSPQLATL ADEVSASLAK QGL
Molecular Weight: 42.1kD (403aa) confirmed by MALDI-TOF
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source
Recombinant, E. coli
Purity
~90% (SDS-PAGE).
Concentration
~1mg/ml
Form
Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 10% glycerol, 0.1M sodium chloride.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
References
1. Cross D., et al. (1993), J Cell Sci, 105: 51-60. 2. Lubke U., et al. (1994), Am. J. Pathol, 145: 175-188.
USBio References
No references available
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