TC-100 is a fully defined medium and does not require supplementation with insect hemolymph. Instead, tryptose broth and fetal bovine serum provide the necessary growth factors. This medium supports the growth of several lepidopteran cell lines including Spodoptera frugiperda. It is specifically used for production of baculoviruses in lepidopteran cell lines. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines.

White to off white, homogenous, free flowing powder

Colorless to light yellow, clear, complete

As Reported

Dissolve 19.07g in 800-900ml of ddH2O stirring gently until completely solubilized. If required, add sodium bicarbonate. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Note: On occasion, this formulation seems reluctant to dissolve. If continuous stirring with warmth has not produced complete dissolution, add 1.0N HCl slowly until solution turns crystal clear with light yellowish tone (pH 4.2-4.5). Let solution cool to RT, then back titrate with 1.0N NaOH to pH 6.2-6.4. Components will remain solubilized.

Store powdered media at RT. Stable for 12 months after receipt. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.