Tenascin is a large glycoprotein that forms disulfide-linked hexamers. Tenascin is made up of a series of repeated structural domains including EGF-like repeats, fibronectin type III repeats, and a region homologous to fibrinogen. Splice variants of tenascin differ in their number of fibronectin type III repeats. Tenascin is expressed in distinctive spatial and temporal patterns in most embryonic tissues at sites of tissue remodeling and cell migration. There is little expression of tenascin in adult tissues except, again, at sites of tissue remodeling and cell migration such as surrounding tumors, in healing wounds, and in branching or damaged blood vessels.
Tenascin binds to several cell-surface receptors and mediates cell-substrate adhesion. Nevertheless, tenascin is frequently referred to as an anti-adhesion molecule because for a variety of cell types the presence of tenascin inhibits adhesion to other ECM proteins present. This effect occurs because the binding of tenascin to certain receptors inhibits cell spreading and thereby blocks the stabilization of adhesion that results from cell preading in vitro assays
Applications
Immunohistochemistry: 2ug/ml on tissues fixed with cold acetone Immunocytochemistry: 2ug/ml on U251 cells Western Blot: 2ug/ml to detect whole tenascin under reducing conditions ELISA: 2x10e10 M endpoint dilution
Affinity constant Kd= 6.37 x 10e9
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Purified human tenascin
Form
Supplied as a liquid in 0.02M PBS, pH 7.6, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes the recombinant fragment of human tenascin containing the fibronectin type III repeats, numbers 1–3
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