Tetratricopeptide repeat protein 5 (TTC5) is a member of a diverse group of functionally distinct proteins that are characterized by containing one or more tetratricopeptide repeats (1). Each motif consists of two anti-parallel a-helices such that tandem arrays of TPR motifs generate a right-handed helical structure with an amphipathic channel that may serve to accommodate the complementary region of a target protein. While the exact function of TTC5 remains unclear, it is thought that the TPR motifs serve to mediate protein-protein interactions such as those seen with protein chaperones HSP70 and HSP90 (2) and some proteins involved in cell stress response signaling pathways such as protein phosphatase 5 (3), suggesting that TTC5 may also function via protein-protein interactions mediated by its TPR motifs.

Suitable for use in Western Blot. Other applications not tested.

Western Blot: 05-1ug/ml Optimal dilutions to be determined by the researcher.

RAW264.7 cell lysate

May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Synthetic peptide TTC5 14 aa near the carboxy terminus of human TTC5 (UniProt # Q8N0Z6).

Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.|

Purified by immunoaffinity chromatography.|

Recognizes human TTC5. Species Crossreactivity: mouse.