Thioglycollate Culture Medium is used for the cultivation of anaerobic, microaerophilic and aerobic microorganisms and for determining the presence of bacteria in normally sterile materials. Sulfhydryl compounds and low concentrations of agar, alone or in combination, are used to culture anaerobic organisms in a fluid broth under aerobic conditions. The use of sodium thioglycollate in these broth media is for the neutralization of mercurial preservatives. Sodium thioglycollate aIso lowers the oxidation-reduction potential of the media. Dextrose is added to enhance growth.
Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.
Appearance
Light beige, to beige homogenous, free flowing powder
Solubility
Pale to light yellow-amber, clear to trace haze
Micrococcus luteus (ATCC 9341)
Cultures after incubation at a temperature of 35ºC and oberved after up to 4 days.
Directions per Liter
Dissolve 30.1 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to 1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).
Storage and Stability
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
| Enzymatic Digest of Casein 17 |
| Soy Peptone 3 |
| Sodium Chloride 2.5 |
| Dextrose 6 |
| Sodium Thioglycollate 0.5 |
| Sodium Sulfite 0.1 |
| L-Cystine 0.25 |
| Agar 0.75 |
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