Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 1:64000 Western Blot: 1-3ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-SPEITTEDIKSAFAP of human TIAL1 & TIA. Species sequence homology: mouse, canine and bovine.
Form
Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human TIAL1 & TIA.
References
1. Yeowell HN, et al. TIA nuclear proteins regulate the alternate splicing of lysyl hydroxylase 2. The Journal of investigative dermatology 2009 Jun 129 (6): 1402-11.