Genomic DNAs are isolated from over 100 different human and fetal normal tissues, human diseased and tumor tissues, mouse, rat, monkey, and plant tissues. Our genomic DNAs are provided ready to use for SNP analysis, Southern Blotting, and PCR. United States Biological offers almost any genomic DNAs from human, mouse, rat, monkey, and plant tissues; no longer will you need to acquire tissues and isolate genomic DNA by yourself.
Tissue genomic DNAs are isolated from a wide variety of tissues. The genomic DNAs are isolated from tissues by proprietary modified guanidine thiocyanate techniques. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0 (detected in 10mM Tris Cl, pH 7.5). Contamination by RNA, polysaccharides and proteoglycans has been effectively eliminated.
Quality Control
1. The quality and purity of genomic DNA were tested by spectrophotometer and electrophoresis. A260/280 is between 1.8-2.0. (Detected in TE, pH 8.0) 2. RNAse treatment to ensure there is no RNA contamination. 3. Genomic DNA was successfully digested by Hind III. 4. A specific fragment of actin gene, ~800bp, was successfully amplified from the genomic DNA.
Features
Genomic DNA isolated from a wide variety of hard to obtain tissues Decontamination of polysaccharide and proteoglycan Extensive quality control procedures ensure high quality genomic DNA High efficiency in PCR Documentation of tissues' clinical histories available
Applications
Suitable for use in SNP analysis, Southern Blot, PCR Genomic DNA library construction, Profiling study in gene expression.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
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