Tissue genomic DNAs are isolated from a wide variety of tissues. The genomic DNAs are isolated from tissues by proprietary modified guanidine thiocyanate techniques. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0 (detected in 10mM Tris Cl, pH 7.5). Contamination by RNA, polysaccharides and proteoglycans has been effectively eliminated.
Features
• Genomic DNA isolated from a wide variety of hard to obtain tissues • Decontamination of polysaccharide and proteoglycan • Extensive quality control procedures ensure high quality genomic DNA • High efficiency in PCR
Applications
Suitable for use in SNP analysis, Southern Blot and PCR Genomic DNA library construction, Profiling study in gene expression.
Quality Control
1. Tested by spectrophotometer and electrophoresis. A260/280 = 1.8-2.0 (detected in 10mM Tris-Cl, pH 7.5) 2. RNase treatment to ensure the RNA contamination is eliminated. 3. HindIII digestion test: Successful. 4. PCR test: A specific fragment of actin gene, about 800bp, was successfully amplified from the genomic DNA.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Form
Supplied as a liquid in 1X TE buffer (10mM Tris, pH 8.0, 1mM EDTA).
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
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