Tissue Protein Lysate produced under consistent processing conditions to ensure intact structure and original nature. Total protein is prepared from whole-tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.
Applications
• Electrophoresis, Western Blotting and Immunoprecipitation • Enzymatic Activity • Protein-DNA Interaction • Protein-Protein Interaction • Tissue-Specific Expression Identification
Additional Data Supplied: All tissue protein samples are supplied with verified donor age and sex.
Preparation
Total protein is prepared from whole-tissue homogenates, immediately frozen in liquid nitrogen after excision, and then stored at -70°C.
Quality Control
1. The isolated protein pattern on SDS-PAGE gel is visualized by Coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western analyzed by either GAPDH or beta-actin antibody, and the expression level is consistent with each lot.
Storage and Stability
For extended storage, keep frozen at -80°C in working aliquots. For continuous use, stored at 2-8°C. Repeated freezing and thawing is not recommended.
Form
Supplied as a frozen liquid in HEPES, pH 7.9, MgCl2, KCl, EDTA, sucrose, glycerol, sodium deoxycholate, NP-40 and a cocktail of protease inhibitors.
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
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