TLR4 has been demonstrated to act as a receptor for LPS on human monocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence of the MD-2 molecule. TLR4 is weakly expressed by resting cells, but is upregulated following stimulation with LPS.
Applications
Suitable for use in Flow Cytometry, Functional Assays, and Immunoprecipitation. Other applications not tested.
Recommended Dilution
Flow Cytometry: 1:10-1:50; 10ul labels 10e6 cells in 100ul Optimal dilutions to be determined by the researcher.
Hybridoma
Sp2/0 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Ba/F3 cell line expressing TLR4.
Form
Supplied as a liquid in PBS. No preservative added.
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes human Toll like receptor 4 (TLR4). Species Crossreactivity: Rhesus monkey, guinea pig, porcine, canine and bovine
TDS
NCBI
UniProt
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