TLRs are highly conserved proteins which play a key role in activation of innate immunity, through the recognition of distinct molecular patterns expressed by infectious agents. TLR7 recognizes and interacts with single-stranded RNA (ssRNA) derived from viruses such as influenza , HIV-1 (human immunodeficiency virus type I) and Sendai, and some siRNAs, resulting in the activation of the MyD88/interleukin-1 receptor-associated kinase (IRAK) signaling pathway, and the production of proinflammatory signaling molecules.

Suitable for use in ELISA, Flow Cytometry and Western Blot. Other applications not tested.

Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Recombinant protein corresponding to aa451-500 from human TLR7, expressed in E. coli.

Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 10% glycerol.

Purified by Protein A affinity chromatography from tissue culture supernatant.

Recognizes human TLR7.