DNA Topoisomerase 2-alpha, EC=5.99.1.3, Topoisomerase (DNA) II alpha 170kDa
DNA topoisomerase 2 catalyzes the transient breaking and rejoining of double stranded DNA during events such as DNA synthesis and transcription. Two forms of this enzyme exist, alpha and beta. DNA topoisomerase 2 alpha (TOP2A) is localized to chromosome 17 and the beta gene is localized to chromosome 3. At least 4 isoforms of TOP2A are produced by alternate splicing.
Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1-2ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide corresponding to aa1466-1480, RKPSTSDDSDSNFEK to human DNA topoisomerase 2-alpha.
Form
Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% Thimerosal, 0.05% sodium azide. Reconstitute with 100ul of sterile dH2O.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human DNA topoisomerase 2-alpha at 174kD. Species Crossreactivity: rat.
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