Applications
Suitable for use in ELISA, Western Blot and Immunocytochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 1:1600 Immunocytochemistry: 1:800 Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS, 10mg/ml BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic analog of the carboxyl terminal of rat transforming growth factor alpha (rTGF alpha) covalently attached onto a carrier protein.
Form
Supplied as a lyophilized powder. Reconstitute with 100ul sterile PBS, 10mg/ml BSA.
Specificity
rRecognizes TGF alpha (Human), TGF alpha (rat), and EGF (human). Does not react to EGF (mouse), Atrial Natriuretic Peptide (human), ACTH (human, 1-39), Calcitonin (human), PTH (bovine, 1-84), Somatostatin and Vasoactive Intestinal Peptide.