Applications
Suitable for use in ELISA, Western Blot and Immunocytochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 1:3000 Immunocytochemistry: 1:1000-1:5000 Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Peptide analog of the carboxyl terminal of the TGF-alpha covalently attached onto a carrier protein.
Form
Supplied as a lyophilized powder. Reconstitution with 0.1ml of 10mg/ml BSA in PBS.
Specificity
Recognizes to TFG-alpha. Does not react to Epidermal Growth Factor (Human). Specific for the COOH terminal of the TGF-alpha.