Protransforming Growth Factor alpha, TGF a, TGF α, TGF-alpha, TGFalpha, Transforming Growth Factor, Transforming Growth Factor, alpha
TGF-alpha is an EGF-related polypeptide growth factor that signals through the EGF receptor, and stimulates the proliferation of a wide range of epidermal and epithelial cells. It is produced by monocytes, keratinocytes, and various tumor cells. TGF-alpha induces anchorage-independence transformation in cultured cells. Human, murine and rat TGF-alpha are cross-species reactive. TGFalpha (aa 50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.
Applications
Suitable for use in ELISA and Western Blot. Other applications have not been tested.
Recommended Dilutions
ELISA (Sandwich): 0.25-1ug/ml (using 100ul/well antibody solution) in conjunction with T8250-03C as a capture antibody, allows the detection of at least 0.2-0.4ng/well of recombinant human TGFa. Western Blot: 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant human TGFa is 1.5-3ng/lane, under either reducing or non-reducing conditions. Optimal working dilutions to be determined by researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to human TGF-alpha, expressed in E. coli.
Form
Supplied as a lyophilized powder from PBS, pH 7.4. No preservative added. Labeled with Biotin. Reconstitute with sterile PBS, 0.1% BSA to a concentration of 0.1-1mg/ml.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human Transforming Growth Factor (TGFa).
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