Transglutaminases are a family of structurally and functionally related enzymes that stabilize protein assemblies through the formation of gamma glutamyl epsilon lysine crosslinks. They all have the same aa sequence at their active sites and their activity is calcium dependent.
Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilutions
Western Blot: 0.1-0.2ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile TBS, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Purified, Sf 21-derived, recombinant human Transglutaminase 7.
Form
Supplied as a lyophilized powder from PBS, 50ug/1ug BSA. Labeled with Biotin. Reconstitute with sterile TBS, pH 7.3, 20mM Trizma, 150mM sodium chloride, 0.1% BSA.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human Transglutaminase 7.
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