Applications
Suitable for use in ELISA, Immunoprecipitation, Immunohistochemistry and Western Blot. Other applications have not been tested.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Hybridoma
Hybridization of Sp2/0 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Human cTnI peptide.
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography from ascites.
Specificity
Recognizes free cardiac troponin I (cTnI) and cTnI forming complexes with other troponin components. Not affected by heparin and phosphorylation. Recognizes an epitope located between amino acid residues 1 and 23 of cTnI. No cross-reactivity with skeletal muscle troponin I.
TDS
NCBI
UniProt
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