Trypsin is a pancreatic serine protease with substrate specificity based upon positively charged lysine and arginine side chains. Trypsin is inhibited by organophosphorous compounds such as diisopropylfluorophosphate and porcine-derived inhibitors. Used in protein sequencing and tissue disassociation.
Manufactured using Trypsin 1:250 (Porcine), Cat# T8670
Source: Porcine pancreas Trypsin: ≥ 250u/mg Chymotrypsin: ≥ 40u/mg Endotoxin (LAL): ≤ 10Eu/ml Porcine Parvovirus (Hemagglutination, PCR): None Detected E. coli: None Detected
Unit Definition
Trypsin: The amount of enzyme which causes an increase in absorbance at 253nm of 0.003 per minute at 25°C
Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Directions per Liter
Dissolve 12.4 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).
Storage and Stability
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.
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