b-Tubulin III belongs to the tubulin family. Tubulin is the major constituent of microtubules. It forms dimers of alpha and beta chains. One mole of tubulin binds to two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. b-Tubulin III contains a highly acidic C-terminal region which can bind cations such as calcium. It is abundant in the central and peripheral nervous systems.
Applications
Suitable for use in ELISA, Western Blot, Immunohistochemistry and Flow Cytometry. Other applications not tested.
Recommended Dilutions
ELISA: 0.05-0.2ug/ml Western Blot: 0.5-1ug/ml Immunohistochemistry: 5-10ug/ml Flow Cytometry: 1-3ug per 1x10e6 cells Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide corresponding to aa150-200 of human b-tubulin III, conjugated to KLH. (Swiss Prot: Q13509). Species Sequence Homology: bovine, chicken, Cynomolgus monkey, African clawed frog, and disc abalone.
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 0.02% sodium azide. Reconstitute with 80ul sterile ddH2O.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes endogenous levels of human beta-tubulin III protein. Species Crossreactivity: mouse and rat.
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