Suitable for use in Immunocytochemistry and Flow Cytometry. Other applications not tested.

Flow Cytometry: 1-3ug/ml using intracellular staining. Optimal dilutions to be determined by the researcher.

Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Peptide (C) 441-448 coupled to maleimide-activated keyhole limpet hemocyanin via cysteine added to the N-terminus of the neuron-specific peptide.

Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).

Purified

Recognizes C-terminal peptide sequence ESESQGPK (aa441-448) of neuron-specific human betaIII-tubulin. Species Crossreactivity: canine, mouse, rat and porcine.