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T9155-04C Rabbit Anti-Tubulin, beta (Beta Tubulin, Tubulin beta Polypeptide, Tubulin beta-2 Chain, OK/SW-cl.56, TUBB, TUBB Protein, TUBB 1, TUBB1, TUBB 2, TUBB2, TUBB 5, TUBB5, XXbac-BCX111D4.2-001) (HRP)

Specifications
References
Clone Type
Monoclonal
Host
Rabbit
Source
Human
Swiss Prot
P07437
Conjugate
HRP
Isotype
IgG
Clone Number
10E54(9F3)
Grade
Purified
Applications
WB
Crossreactivity
Bo Hu Mk Mo Rt Ze
Gene ID
203068
Shipping Temp
Blue Ice
Storage Temp
-20°C

The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with alpha/beta-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. gamma-tubulin is necessary to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competi-tive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins (1).

Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to the N-terminus of human beta-tubulin. Species Sequence Homology: chicken (100%)
Form
Supplied as liquid in PBS, pH 7.4, 2mg/ml BSA, 50% glycerol. Labeled with horseradish peroxidase (HRP).
Purity
Purified
Specificity
Recongnizes endogenous levels of human total Beta-tubulin protein and does not cross-react with recombinant alpha-tubulin. Species crossreactivity: mouse, rat, monkey, bovine, zebrafish.
References
1. Westermann, S. and Weber, K. (2003) Nat. Rev. Mol. Cell Biol. 4, 938-947.
USBio References
No references available
Conjugates
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