Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
ELISA (Peptide): 1:32000 Western Blot: 0.001-0.01ug/ml Immunohistochemistry (FFPE): 1-2ug/ml Human Cerebellum shows strong staining of axonal bundles through the granular layer. Requires HIER using sodium citrate buffer pH6.0 prior to staining. Immunohistochemistry (FFPE): 3.75ug/ml Human Cortex Requires HIER using sodium citrate buffer pH6.0 prior to staining. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after reciept. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-DPSKDRAAKRLSLES of human TPPP.
Form
Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human TPPP. Species Crossreactivity: mouse and rat.
References
1. Ovádi J, Orosz F. An unstructured protein with destructive potential: TPPP/p25 in neurodegeneration.|BioEssays : news and reviews in molecular, cellular and developmental biology 2009 Jun 31 (6): 676-86.