Tumor Necrosis Factor alpha

Clone Type

Monoclonal

Host

Rat

Source

Mouse

Isotype

IgG1,k

Clone Number

3H3094 (MP6-XT22)

Grade

Purified

Applications

E FC IHC N

Crossreactivity

Ch Mo

Shipping Temp

Blue Ice

Storage Temp

-20°C

Notes

Preservative Free

TNF alpha is a 17kD cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4+ T cells. TNF alpha forms dimers and trimers, and is also found as a 26KD integral membrane protein. Mouse TNF alpha has cytolytic activity against many tumor cell lines, and has an important immuno-regulatory role.

Applications

Suitable for use in ELISA, Flow Cytometry, Immunohistochemistry and Functional Assays. Other applications not tested.

Recommended Dilution

ELISA: ≤2ug/ml Immunohistochemistry (frozen): see references 13-19 Flow Cytometry: see references 6-12 Neutralization: see references 1 and 22-24 Optimal dilutions to be determined by the researcher.

Storage and Stability

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunogen

Recombinant protein corresponding to mouse TNF alpha expressed in E. coli.

Form

Supplied as a liquid in PBS, pH 7.4. No preservatives added.

Purity

Purified

Specificity

Recognizes mouse Tumor Necrosis Factor alpha (TNFa). |Species Crossreactivity: chicken

References

1. Abrams JS, et al., Immunol Rev. 1992;127:5-24. (ELISA-Detection, Neut) 2. Heinzel FP, et al. J Immunol. 1996;157:4521-8. (ELISA-Capture) 3. Bordmann G, et al., Immunology. 1998;94:35-40. (ELISA-Capture) 4. Abrams JS. et al.. Curr Protoc Immunol. 2001;6.20:1-15. (ELISA-Detection) 5. Favre N, J Immunol Methods. 1997;204:57-66. (ELISPOT-Capture) 6. Tripp RA, J Virol. 1999;73:7099-107. (FC) 7. Asseman C, J Exp Med. 1999;190:995-1003. (FC) 8. Satoh M, Transplantation. 2007;83:1085-92. (FC) 9. Hamada H, J Immunol. 2009;182:3469-81. (FC) 10. Voß EV, Neurobiol Dis. 2012;45:519-28. (FC) 11. Le Huu D, et al., Blood. 2013;121:3274-83. (FC) 12. Nakamura T, et al.,. Am J Transplant. 2015 May 5. doi: 10.1111/ajt.13276. [Epub ahead of print]. (FC) 13. Hunter CA, et al. J Infect Dis. 1994;170:939-45. (IHC-FS) 14. Sunnemark D, et al, Scand J Immunol. 1996;44:421-9. (IHC-FS)15. Marinova-Mutafchieva L, et al. Clin Exp Immunol. 1997;107:507-12. (IHC-FS)16. Whiteland JL, J Immunol Methods. 1997;210:103-8. (IHC-FS, IHC-PS) 17. Issazadeh S, et al. J Clin Invest. 2000;105:1109-16. (IHC-FS) 18. Jacobs M, et al. . 2000;100:494-501. (IHC-FS) 19. Williams RO, et al.,. J Immunol. 2000;165:7240-5. (IHC-FS) 20. Sanders EJ, et al., Cell Death Differ. 1997;4:188-99. (ICC, Chicken Reactivity) 21. Wride MA, et al., Dev Dyn. 1993;198:225-39. (ICC, WB, Chicken Reactivity) 22. Scanga CA, et al.,. Infect Immun. 1999;67:4531-8. (Neut) 23. Via CS, et al.,. J Immunol. 2001;167:6821-6. (Neut) 24. Zhou R, J Immunol. 2007;178:4548-56. (Neut) 25. Muir BW, Barden MC, Rylatt DB, Maeji NJ, Hillyard CJ, Gorse AJ, et al, inventors. Bio-Layer Pty Limited, assignee. Use of metal complexes. United States patent US 8,168,445 B2.2012 May 1. (Multiplex-Detection)

USBio References

No references available

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T9160-13K Rat Anti-Tumor Necrosis Factor alpha (TNFa, TNF-alpha, Tumor Necrosis Factor Ligand Superfamily Member 2, TNF-a, Cachectin, TNF, TNFA, TNFSF2) (Azide Free, Low Endotoxin)

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