Suitable for use in Flow Cytometry, Western Blot and FLISA. Other applications not tested.

Functional Assays: 1:50, used for inhibition of the biological activity of TNF-alpha. Western Blot: 1:50 Optimal ddilutions to be determined by the researcher.

Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

BALB/c mice with huTNF, fusion of spleen cells with NSO myeloma cells

Supplied as a liquid in PBS, 1% BSA, 0.02% sodium azide. Labeled with fluorescein isothiocyanate (FITC).

Purified by Protein G affinity chromatography.

Recognizes the free soluble (17kD) and membrane (26kD) human TNF-alpha. The antibody inhibits the biological activity of both forms. It does not react with receptor bound TNF-alpha.