Suitable for use in ELISA and Immunofluorescence. Other applications not tested.

ELISA:1:32000 Immunofluorescence: 50ug/ml stains the islets in Mouse Pancreas after Citrate antigen retrieval. Optimal dilutions to be determined by the researcher.

May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Corresponding peptide is available for Peptide Blocking studies. See U0893-50-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.

Synthetic peptide corresponding to NCTVSEEPTGID, from the internal region of human UNC5C.

Supplied as a liquid in Tris, pH 7.3, 0.5% BSA, 0.02% sodium azide.

Purified by peptide affinity chromatography.

Recognizes human UNC5C. Species sequence homology: mouse, canine.