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U1500-70E Rabbit Anti-ULK1 (Serine/Threonine-protein Kinase ULK1, Unc-51-like Kinase 1, KIAA0722)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
IHC WB
Crossreactivity
Hu Mk Mo
Accession #
NP_003556
Shipping Temp
Blue Ice
Storage Temp
-20°C

ULK1 belongs to the Ser/Thr protein kinase family. It interacts with GABARAP and GABARAPL2 and is important for vesicle transport and axonal elongation in mammalian neurons. It is involved in starvation-induced autophagy, thus maintaining homeostasis. It is colocalized in many kind of neurons such as pyramidal cells of the hippocampus, mitral cells of the olfactory bulb, and Purkinje cells of the cerebellum.ulK1 plays an essential role in neurite extension of cerebellar granule cells. It is expressed in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung.

Applications
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 1-3ug/ml Immunohistochemistry (paraffin): 10ug/ml Optimal dilutions to be determined by the researcher.
Positive Control
Raji and RAW cell lysate
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to aa320-370 of human ULK1.
Form
Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human ULK1/Unc-51. Species Crossreactivity: monkey, mouse. Species sequence homology: bovine, canine, rat.
References
1. Young, AR. et al. Autophagy. 3:54-56 (2007).
USBio References
No references available
Conjugates
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