Suitable for use in Peptide ELISA and Western Blot. Other applications have not been tested.
Recommended Dilutions
Western Blot: 0.3-1ug/ml ~90kD band observed in Human Skeletal Muscle lysates (calculated MW of 104kD according to NP_775259.1). Optimal dilutions to be determined by the researcher.
Titer
Peptide ELISA: 1:2000
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to C-RSDKLRQKIFKER, from the internal region of human UNC45B (NP_775259.1; NP_001028748.1).
Form
Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Purity
Purified by peptide affinity chromatography.
Specificity
Recognizes human UNC45B. Expected to recognize both reported isoforms (NP_775259.1and NP_001028748.1). Species Sequence Homology: mouse, rat and canine
References
1. Landsverk ML, Li S, Hutagalung AH, Najafov A, Hoppe T, Barral JM, Epstein HF. The UNC-45 chaperone mediates sarcomere assembly through myosin degradation in Caenorhabditis elegans. J Cell Biol. 2007 Apr 23;177(2):205-10. Epub 2007 Apr 16.
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