Applications
Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution
Immunohistochemistry (formalin-fixed paraffin): 1:100 (ABC, DAB) Incubate 1 hour at RT. Optimal dilutions to be determined by the researcher.
Positive Control
Rat piriform cortex
AA Sequence
Asp-Asn-Pro-Ser-Leu-Ser-Ile-Asp-Leu-Thr-Phe-His-Leu-Leu-Arg-Thr-Leu-Leu-Glu-Leu-Ala-Arg-Thr-Gln-Ser-Gln-Arg-Glu-Arg-Ala-Glu-Gln-Asn-Arg-Ile-Ile-Phe-Asp-Ser-Val-NH2
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide corresponding to human Urocortin.
Form
Supplied as a lyophilized powder. Reconstitute with 50ul sterile ddH2O.
Specificity
Recognizes human Urocortin. Species crossreactivity: Rat Urocortin (100%), Rat Cst-14 (1.94%) Does not react with: human or rat CRF, human, mouse or rat MCH, human, porcine or rat LH-RH, Somatostatin, or human or rat NPY.